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lbcas12a protein  (New England Biolabs)


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    New England Biolabs lbcas12a protein
    Lbcas12a Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lbcas12a+protein/NEBuffer+2/pmc12962081-97-21-17
    Average 99 stars, based on 2971 article reviews
    lbcas12a protein - by Bioz Stars, 2026-09
    99/100 stars

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    Establishment of the <t>RT-ERA/LbCas12a</t> fluorescence assay. (A) Optimization of crRNA3 concentration. The crRNA3 was diluted to five different concentrations 12.5 nM, 25 nM, 50 nM, 100 nM, and 200 nM, and added to the reaction system. The reaction mixtures were incubated for 10, 20, 30, 45 or 60 min and then visualized under UV light. (B) Optimization of the LbCas12a concentration. LbCas12a was diluted to five different concentrations, and each concentration was added to the reaction system. The reaction mixtures were incubated and visualized under UV light.
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    Establishment of the <t>RT-ERA/LbCas12a</t> fluorescence assay. (A) Optimization of crRNA3 concentration. The crRNA3 was diluted to five different concentrations 12.5 nM, 25 nM, 50 nM, 100 nM, and 200 nM, and added to the reaction system. The reaction mixtures were incubated for 10, 20, 30, 45 or 60 min and then visualized under UV light. (B) Optimization of the LbCas12a concentration. LbCas12a was diluted to five different concentrations, and each concentration was added to the reaction system. The reaction mixtures were incubated and visualized under UV light.
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    Establishment of the RT-ERA/LbCas12a fluorescence assay. (A) Optimization of crRNA3 concentration. The crRNA3 was diluted to five different concentrations 12.5 nM, 25 nM, 50 nM, 100 nM, and 200 nM, and added to the reaction system. The reaction mixtures were incubated for 10, 20, 30, 45 or 60 min and then visualized under UV light. (B) Optimization of the LbCas12a concentration. LbCas12a was diluted to five different concentrations, and each concentration was added to the reaction system. The reaction mixtures were incubated and visualized under UV light.

    Journal: Poultry Science

    Article Title: A RT-ERA-CRISPR/Cas12a assay for rapid point-of-care duck hepatitis A virus detection

    doi: 10.1016/j.psj.2025.105316

    Figure Lengend Snippet: Establishment of the RT-ERA/LbCas12a fluorescence assay. (A) Optimization of crRNA3 concentration. The crRNA3 was diluted to five different concentrations 12.5 nM, 25 nM, 50 nM, 100 nM, and 200 nM, and added to the reaction system. The reaction mixtures were incubated for 10, 20, 30, 45 or 60 min and then visualized under UV light. (B) Optimization of the LbCas12a concentration. LbCas12a was diluted to five different concentrations, and each concentration was added to the reaction system. The reaction mixtures were incubated and visualized under UV light.

    Article Snippet: LbCas12a protein was purchased from Synbio Technologies (Suzhou, China).

    Techniques: Fluorescence, Concentration Assay, Incubation

    RT-ERA/LbCas12a-LFA assay establishment. (A) Optimization of the FAM-biotin-conjugated ssDNA probe. (B) Optimization of crRNA3 concentration. (C) Optimization of the LbCas12a concentration. The reaction system of the RT-ERA/LbCas12a-LFA assay, including different crRNA3 concentrations, LbCas12a concentrations and ssDNA reporter concentrations was determined using the cleavage efficiency of the LbCas12a protein.

    Journal: Poultry Science

    Article Title: A RT-ERA-CRISPR/Cas12a assay for rapid point-of-care duck hepatitis A virus detection

    doi: 10.1016/j.psj.2025.105316

    Figure Lengend Snippet: RT-ERA/LbCas12a-LFA assay establishment. (A) Optimization of the FAM-biotin-conjugated ssDNA probe. (B) Optimization of crRNA3 concentration. (C) Optimization of the LbCas12a concentration. The reaction system of the RT-ERA/LbCas12a-LFA assay, including different crRNA3 concentrations, LbCas12a concentrations and ssDNA reporter concentrations was determined using the cleavage efficiency of the LbCas12a protein.

    Article Snippet: LbCas12a protein was purchased from Synbio Technologies (Suzhou, China).

    Techniques: Concentration Assay